CΔ2
(Plasmid #74297)

• Purpose: Mammalian expression of treacle (residues 1176-1270) fused to GFP
• Depositing Lab: Rita Shiang
• Publication: Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Backbone

• Vector backbone: pS65T-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4728
• Total vector size (bp): 4823
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: treacle ribosome biogenesis factor 1
• Alt name: Tcof1
• Species: M. musculus (mouse)
• Insert Size (bp): 95
• Mutation: Expresses residues 1176 - 1270
• Entrez Gene: Tcof1 (a.k.a. treacle)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ecl136II (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: pEGFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the amino acid sequence SGEFCSRRYRGPGIHRI is inserted after residue 1270 of treacle. The depositor noted that this sequence does NOT affect plasmid function.