pJK122
(Plasmid #74295)

• Purpose: Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, cleavable N-terminal NusA fusion
• Depositing Lab: T. J. Kappock
• Publication: Additional Acetobacter aceti 1023 acetic acid resistance (aar) gene constructs (unpublished)

Backbone

• Vector backbone: pET-43.1a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 7194
• Total vector size (bp): 7672
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hypothetical acetic acid resistance protein B
• Alt name: AarB
• Species: Acetobacter aceti 1023
• Promoter: T7
• Tag / Fusion Protein:
  • NusA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PshAI (destroyed during cloning)
• 3′ cloning site: blunt (unknown if destroyed)
• 5′ sequencing primer: Nus-tag primer
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene constructed from synthetic DNA. Sequence contains extra 3' - noncoding region between EcoRI sites.

Produces a form of a protein described as AarB in Fukaya M, Takemura H, Okumura H, Kawamura Y, Horinouchi S, Beppu T. Cloning of genes responsible for acetic acid resistance in Acetobacter aceti. J Bacteriol. 1990 Apr;172(4):2096-104. (PMID 2156811)

Gene reannotated as SixA, on other strand, in Mullins EA, Francois JA, Kappock TJ. A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti. J Bacteriol. 2008 Jul;190(14):4933-40. doi: 10.1128/JB.00405-08. (PMID 18502856)