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Addgene

pAAV-Ef1a-DIO-PBG-WPRE-hGH
(Plasmid #74287)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 74287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-DIO
  • Backbone manufacturer
    K. Deisseroth lab (Stanford)
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PBG (chimeric rabies Glycoprotein)
  • Species
    glycoprotein for rabies virus SAD B19
  • Promoter Ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer EF1a-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Ef1a-DIO-PBG-WPRE-hGH was a gift from Edward Callaway (Addgene plasmid # 74287 ; http://n2t.net/addgene:74287 ; RRID:Addgene_74287)

  • For your References section:

    Three Types of Cortical Layer 5 Neurons That Differ in Brain-wide Connectivity and Function. Kim EJ, Juavinett AL, Kyubwa EM, Jacobs MW, Callaway EM. Neuron. 2015 Dec 16;88(6):1253-67. doi: 10.1016/j.neuron.2015.11.002. Epub 2015 Dec 6. 10.1016/j.neuron.2015.11.002 PubMed 26671462
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