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Addgene

pDA199
(Plasmid #74275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIVL
  • Total vector size (bp) 8620
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pSTL1-dPSTRy(4,3)
  • Species
    S. cerevisiae (budding yeast)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains the complete pSTL1-dPSTRy(4,3). The vector can be cut either PacI or BstBI prior to transformation in yeast. Selection in Leu depleted medium.
For more information, see Aymoz et al. Nat. Comm. 2016.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDA199 was a gift from Serge Pelet (Addgene plasmid # 74275 ; http://n2t.net/addgene:74275 ; RRID:Addgene_74275)

  • For your References section:

    Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters. Aymoz D, Wosika V, Durandau E, Pelet S. Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304. 10.1038/ncomms11304 PubMed 27098003
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