pKanCMV-CX4.3-7aa-mClover3
(Plasmid #74260)

• Purpose: Mammalian expression of C-terminal fusion of mClover3 to CX43
• Depositing Lab: Michael Lin
• Publication: Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.

Backbone

• Vector backbone: pKanCMV
• Backbone manufacturer: Michael Davidson Lab
• Backbone size w/o insert (bp): 3900
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Connexin 43
• Alt name: CX43
• Insert Size (bp): 1146
• Promoter: CAG
• Tag / Fusion Protein:
  • mClover3 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: pshuttle5
• 3′ sequencing primer: pM3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.