pKanCMV-mRuby3-18aa-actin
(Plasmid #74255)

• Purpose: Mammalian expression of N-terminal fusion of mRuby3 to actin
• Depositing Lab: Michael Lin
• Publication: Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.

Backbone

• Vector backbone: pKanCMV
• Backbone manufacturer: Michael Davidson Lab
• Backbone size w/o insert (bp): 3900
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: actin
• Insert Size (bp): 1128
• Promoter: CAG
• Tag / Fusion Protein:
  • mRuby3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: pshuttle5
• 3′ sequencing primer: pM3

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.