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pKanCMV-CX4.3-7aa-mRuby3
(Plasmid #74254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKanCMV
  • Backbone manufacturer
    Michael Davidson Lab
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Mammalian Expression
  • Selectable markers
    none

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 43
  • Alt name
    CX43
  • Insert Size (bp)
    1146
  • Promoter CAG
  • Tag / Fusion Protein
    • mRuby3 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer pshuttle5
  • 3′ sequencing primer pM3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, T38V, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKanCMV-CX4.3-7aa-mRuby3 was a gift from Michael Lin (Addgene plasmid # 74254 ; http://n2t.net/addgene:74254 ; RRID:Addgene_74254)

  • For your References section:

    Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144
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