pNCS-mClover3
(Plasmid #74236)

• Purpose: Constitutive expression of mClover3 in bacteria
• Depositing Lab: Michael Lin
• Publication: Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.

Backbone

• Vector backbone: pNCS
• Backbone size w/o insert (bp): 2916
• Total vector size (bp): 3633
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mClover3
• Insert Size (bp): 717
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7 Xpress Fwd
• 3′ sequencing primer: T7 term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The specific substitutions used in the development of mClover3 relative to its parent Clover are N149Y, G160C, and A206K.

Information for Cloning Grade DNA (Catalog # 74236-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here