pNCS-mRuby3
(Plasmid #74234)

• Purpose: Constitutive expression of mRuby3 in bacteria
• Depositing Lab: Michael Lin
• Publication: Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.

Backbone

• Vector backbone: pNCS
• Backbone size w/o insert (bp): 2916
• Total vector size (bp): 3627
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: May require extra growth time
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mRuby3
• Insert Size (bp): 711
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7 Xpress Fwd
• 3′ sequencing primer: T7 term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The specific substitutions used in the development of mRuby3 relative to its parent mRuby2 are N33R, M36E, K74A, G75D, M105T, C114E, H118N, Q120K, H159D, M160I, S171H, S173N, I192V, L202I, M209T, F210Y, H216V, F221Y, A222S, G223N.

Note that the mRuby3 insert in this plasmid does not contain T38V, like the canonical mRuby3. See publication and full plasmid sequence for more information.