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Addgene

pHAGE-eGFP-Ago2
(Plasmid #74231)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ago2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    924
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Generated by: Etienne Gagnon

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-eGFP-Ago2 was a gift from Carl Novina (Addgene plasmid # 74231 ; http://n2t.net/addgene:74231 ; RRID:Addgene_74231)

  • For your References section:

    Alternative RISC assembly: binding and repression of microRNA-mRNA duplexes by human Ago proteins. Janas MM, Wang B, Harris AS, Aguiar M, Shaffer JM, Subrahmanyam YV, Behlke MA, Wucherpfennig KW, Gygi SP, Gagnon E, Novina CD. RNA. 2012 Nov;18(11):2041-55. doi: 10.1261/rna.035675.112. Epub 2012 Sep 27. 10.1261/rna.035675.112 PubMed 23019594
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