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Addgene

pCI 9.0
(Plasmid #74229)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74229 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.20
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5404
  • Modifications to backbone
    Removal of FLuc2 from pGL4.20 via restriction digest with HindIII and BamHI. Insert Geneart (LifeTechnologies) synthesized 3xFLAGFLuc-P2A-3xFLAGNLucPEST using restriction cloning.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFlag-FLuc-P2A-3xFlag-NLucPEST

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCI 9.0 is a promoterless version of FLuc-P2A-NLucPEST coincidence reporter plasmid. pCI 9.0 contains a 5' MCS region for insertion of a promoter or response element of interest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI 9.0 was a gift from James Inglese (Addgene plasmid # 74229 ; http://n2t.net/addgene:74229 ; RRID:Addgene_74229)

  • For your References section:

    Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit. Schuck BW, MacArthur R, Inglese J. Curr Protoc Neurosci. 2017 Apr 10;79:5.32.1-5.32.27. doi: 10.1002/cpns.27. 10.1002/cpns.27 PubMed 28398644
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