-
Purpose(Empty Backbone) Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepSB1K30
- Backbone size (bp) 2255
-
Modifications to backboneInsertion of BBa_K1362000 into BioBrick cloning site
-
Vector typeBacterial Expression, Synthetic Biology
- Promoter T7-lac
-
Tags
/ Fusion Proteins
- Npu DnaE C-intein (N terminal on backbone)
- Npu DnaE N-intein (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer VF2
- 3′ sequencing primer VR (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byH. D. Mootz, University of Münster
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCIRCNpu was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 74226 ; http://n2t.net/addgene:74226 ; RRID:Addgene_74226) -
For your References section:
Backbone circularization of Bacillus subtilis family 11 xylanase increases its thermostability and its resistance against aggregation. Waldhauer MC, Schmitz SN, Ahlmann-Eltze C, Gleixner JG, Schmelas CC, Huhn AG, Bunne C, Buscher M, Horn M, Klughammer N, Kreft J, Schafer E, Bayer PA, Kramer SG, Neugebauer J, Wehler P, Mayer MP, Eils R, Di Ventura B. Mol Biosyst. 2015 Nov 10;11(12):3231-43. doi: 10.1039/c5mb00341e. 10.1039/c5mb00341e PubMed 26434634
Map uploaded by the depositor.
