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Addgene

pLenti puro HA-Ubiquitin
(Plasmid #74218)

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Item Catalog # Description Quantity Price (USD)
Plasmid 74218 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pLenti puro
  • Backbone manufacturer
    synthesized combination of lentiviral elements in pUC19
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin
  • Alt name
    UBB
  • Alt name
    Ubiquitin B
  • Species
    H. sapiens (human)
  • Mutation
    Single ubiquitin coding sequence (full length UBB has three repeating ubiquitin coding sequences)
  • GenBank ID
  • Entrez Gene
    UBB (a.k.a. HEL-S-50)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti puro HA-Ubiquitin was a gift from Melina Fan (Addgene plasmid # 74218 ; http://n2t.net/addgene:74218 ; RRID:Addgene_74218)
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