pMZ377
(Plasmid
#74214)
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PurposeCombined sgRNA/Cas9 vector with LEU2 marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMZ374
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Vector typeYeast Expression, CRISPR
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCas9
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MutationSilent muation of the CspCI site
- Promoter adh1
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer GGT TTT CAG TAT AAT GTT ACA TGC G (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namerrk1:sgRNA
- Promoter rrk1
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer TGT AAA ACG ACG GCC AGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMZ377 was a gift from Mikel Zaratiegui (Addgene plasmid # 74214 ; http://n2t.net/addgene:74214 ; RRID:Addgene_74214)
