pLentiblast-Paxillin-mTurqoise
(Plasmid #74206)

• Purpose: Lentiviral expression of Paxillin-mTurqoise
• Depositing Lab: Jayanta Debnath
• Publication: Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590. Epub 2016 Feb 22.

Backbone

• Vector backbone: pLenti PGK GFP Blast (w510-5)
• Backbone manufacturer: Addgene plasmid #19069
• Backbone size w/o insert (bp): 7563
• Total vector size (bp): 9950
• Modifications to backbone: Insert was digested with BglII/PspXI and ligated into vector digested with BamHI/SalI.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Paxillin
• Alt name: PXN
• Species: Chicken
• Insert Size (bp): 1677
• Tag / Fusion Protein:
  • mTurqoise (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: PGKforw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mTurqoise-Paxillin was cloned from mTurquoise-Paxillin-22 from Michael Davidson (Addgene plasmid # 55573). The backbone: pLenti PGK GFP Blast (w510-5) was a gift from Eric Campeau (Addgene plasmid # 19069).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry