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PurposeFor packaging hDot1Lmut (catalytic activity dead) to retrovirus.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74174 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCB
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Vector typeRetroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehDot1L
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SpeciesH. sapiens (human)
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Insert Size (bp)5200
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MutationhDot1L 163 GSG to RCR mutation*
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Entrez GeneDOT1L (a.k.a. DOT1, KMT4)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
*Addgene quality control sequence found T100M as well as expected mutations. This mutation is not known to affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCB-hDot1Lmut was a gift from Yi Zhang (Addgene plasmid # 74174 ; http://n2t.net/addgene:74174 ; RRID:Addgene_74174) -
For your References section:
hDOT1L links histone methylation to leukemogenesis. Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, Zhang Y. Cell. 2005 Apr 22;121(2):167-78. 10.1016/j.cell.2005.02.020 PubMed 15851025