-
PurposeFor packaging hDot1Lwt to retrovirus.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 74173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMSCB
-
Vector typeRetroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namehDot1L
-
SpeciesH. sapiens (human)
-
Insert Size (bp)5200
-
Entrez GeneDOT1L (a.k.a. DOT1, KMT4)
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MSCB-hDot1Lwt was a gift from Yi Zhang (Addgene plasmid # 74173 ; http://n2t.net/addgene:74173 ; RRID:Addgene_74173) -
For your References section:
hDOT1L links histone methylation to leukemogenesis. Okada Y, Feng Q, Lin Y, Jiang Q, Li Y, Coffield VM, Su L, Xu G, Zhang Y. Cell. 2005 Apr 22;121(2):167-78. 10.1016/j.cell.2005.02.020 PubMed 15851025