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Purpose(Empty Backbone) Lentiviral vector for expression of heterologous genes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAIP
- Backbone size (bp) 7826
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Vector typeMammalian Expression, Lentiviral
- Promoter SFFV
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAIP was a gift from Jeremy Luban (Addgene plasmid # 74171 ; http://n2t.net/addgene:74171 ; RRID:Addgene_74171) -
For your References section:
TRIM5 is an innate immune sensor for the retrovirus capsid lattice. Pertel T, Hausmann S, Morger D, Zuger S, Guerra J, Lascano J, Reinhard C, Santoni FA, Uchil PD, Chatel L, Bisiaux A, Albert ML, Strambio-De-Castillia C, Mothes W, Pizzato M, Grutter MG, Luban J. Nature. 2011 Apr 21;472(7343):361-5. doi: 10.1038/nature09976. 10.1038/nature09976 PubMed 21512573