pVEX-CC1
(Plasmid #74170)

• Purpose: protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria
• Depositing Lab: Trina Schroer
• Publication: Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Backbone

• Vector backbone: pVEX
• Backbone manufacturer: Nature Technology Corporation
• Backbone size w/o insert (bp): 4241
• Total vector size (bp): 5240
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DCTN1
• Alt name: Dynactin p150Glued
• Species: G. gallus (chicken)
• Insert Size (bp): 999
• Entrez Gene: DCTN1 (a.k.a. Chip150)
• Promoter: Inducible Tac promoter
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CGTGCCATGGAGGAAGAAAATCTGCGTTCC
• 3′ sequencing primer: CCGGGATCCTTACTGCTGCTGCTTCTCTGC

Resource Information

• Supplemental Documents:
  • pVEX vector map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry