pUHD 10.3-human MIZ1 delta POZ
(Plasmid #74160)

• Purpose: Tet inducible expression of MIZ1 delta POZ
• Depositing Lab: Martine Roussel
• Publication: Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi: 10.1016/j.ccell.2015.12.003.

Backbone

• Vector backbone: pUHD
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MIZ1 delta POZ
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Mutation: delta POZ domain (first 109 aa)
• Entrez Gene: ZBTB17 (a.k.a. MIZ-1, ZNF151, ZNF60, pHZ-67)
• Promoter: CMV2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: EBV-Rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry