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PurposeExpress SMCR8 HA tagged in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6
- Total vector size (bp) 7689
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMCR8
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SpeciesH. sapiens (human)
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Entrez GeneSMCR8 (a.k.a. DENND8A)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV
- 3′ sequencing primer hGH polyA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV6 SMCR8 HA was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74158 ; http://n2t.net/addgene:74158 ; RRID:Addgene_74158)