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pCDNA3 HA C9ORF72 op
(Plasmid #74156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Total vector size (bp) 6833
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C9ORF72
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1446
  • Mutation
    optimized sequence
  • Entrez Gene
    C9orf72 (a.k.a. ALSFTD, DENND9, DENNL72, FTDALS, FTDALS1)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HIND3 (not destroyed)
  • 3′ cloning site XHO1 (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer BGH polyA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

C9ORF72 sequence has been optimized, meaning it encodes same amino acid sequence than natural C9ORF72 (human) but with optimized nucleotide sequence to remove negative RNA element and rare codons.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3 HA C9ORF72 op was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 74156 ; http://n2t.net/addgene:74156 ; RRID:Addgene_74156)
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