Xlim-1 (Lhx1) 3m
(Plasmid
#74136)
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PurposeThis is a mutant of Xlim-1/Lhx1 with one point mutation in each of the two LIM domains. Cloned in XbetaM. To prepare sense RNA: SalI/SP6.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74136 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneXbetaM
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Vector typeexpression in frog embryo
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelhx1 3m
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Alt nameXlim-1 3m
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SpeciesX. laevis (frog)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cloned in this lab
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Xlim-1 (Lhx1) 3m was a gift from Igor Dawid (Addgene plasmid # 74136 ; http://n2t.net/addgene:74136 ; RRID:Addgene_74136) -
For your References section:
Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus. Taira M, Otani H, Saint-Jeannet JP, Dawid IB. Nature. 1994 Dec 15;372(6507):677-9. 10.1038/372677a0 PubMed 7990959