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Addgene

FtsZ-MEEVF
(Plasmid #74126)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCA24N
  • Backbone manufacturer
    NBRP-E.coli at NIG
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FtsZ-MEEVF
  • Species
    E. coli
  • Insert Size (bp)
    912
  • Promoter T5-lac
  • Tag / Fusion Protein
    • C-terminal linker sequence with the MEEVF tag (sequence G G S G S G S S M E E V F) (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCGTAATAGCGAAGAGGCCCG
  • 3′ sequencing primer cattactggatctatcaacaggag
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original FtsZ expression construct was a kind gift from Jie Xiao (Addgene plasmid # 49764).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FtsZ-MEEVF was a gift from Lynne Regan (Addgene plasmid # 74126 ; http://n2t.net/addgene:74126 ; RRID:Addgene_74126)
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