pJKW 0643
(Plasmid #74121)

• Purpose: Photinus pyralis luciferin sulfotransferase LST - pHis8-4
• Depositing Lab: Jing-Ke Weng
• Publication: Fallon et al Biochemistry. 2016 May 26.

Backbone

• Vector backbone: pHis8-4
• Backbone manufacturer: Produced in Jing-Ke Weng lab
• Backbone size w/o insert (bp): 5350
• Total vector size (bp): 6456
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Photinus pyralis firefly luciferin sulfotransferase
• Alt name: LST
• Species: Photinus pyralis
• Insert Size (bp): 1113
• GenBank ID: KX097998
• Promoter: T7
• Tag / Fusion Protein:
  • 8x-His, TEV protease site (ENLYFQ) (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • Plasmid genbank file.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Expressed robustly in E.coli. Catalyzes sulfonation / desulfonation of firefly luciferin / sulfoluciferin with PAPS / PAP.