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Addgene

pISceI-myl7:MBIC5-mCer3
(Plasmid #74117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 5612
  • Vector type
    Mammalian Expression ; mRNA generation for microinjection.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBIC5-mCer3
  • Species
    Synthetic
  • Insert Size (bp)
    1521
  • Promoter SP6
  • Tag / Fusion Protein
    • mCerulean 3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pISceI-myl7:MBIC5-mCer3 was a gift from Michael Tsang (Addgene plasmid # 74117 ; http://n2t.net/addgene:74117 ; RRID:Addgene_74117)

  • For your References section:

    A genetically targetable near-infrared photosensitizer. He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St Croix CM, Tsang M, Bruchez MP. Nat Methods. 2016 Mar;13(3):263-268. doi: 10.1038/nmeth.3735. Epub 2016 Jan 25. 10.1038/nmeth.3735 PubMed 26808669
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