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PurposeEnhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR8
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2818
- Total vector size (bp) 3548
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Vector typeGateway TOPO Cloning
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameminiSOG(Q103L)
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Insert Size (bp)730
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Tag
/ Fusion Protein
- PH (N terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCZGY2849 was a gift from Andrew Chisholm (Addgene plasmid # 74112 ; http://n2t.net/addgene:74112 ; RRID:Addgene_74112) -
For your References section:
Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG. Xu S, Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271. 10.1038/srep21271 PubMed 26861262
