pRGD-TcR
(Plasmid
#74110)
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PurposePlasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 74110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSL15
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Backbone manufacturerAlexeyev, M.F., BioTechniques 18:52-56, 1995
- Total vector size (bp) 2867
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Vector typeBacterial vector based on pMB1 replicon
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametetA(C)
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Insert Size (bp)1368
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MutationC-to-T transition at pos. no. 1049 of tetA(C) leading to T350I muation in TetA(C)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe tetracycline resistance gene is derived from pBR322 (Bolivar, F. et al., Gene 2:95-113, 1977)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRGD-TcR was a gift from Hans-Martin Fischer (Addgene plasmid # 74110 ; http://n2t.net/addgene:74110 ; RRID:Addgene_74110) -
For your References section:
Versatile vectors for efficient mutagenesis of Bradyrhizobium diazoefficiens and other Alphaproteobacteria. Ledermann R, Strebel S, Kampik C, Fischer HM. Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15. 10.1128/AEM.04085-15 PubMed 26921431
Map uploaded by the depositor.
