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Addgene

pRGD-SmR
(Plasmid #74109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSL15
  • Backbone manufacturer
    Alexeyev, M.F., BioTechniques 18:52-56, 1995
  • Total vector size (bp) 2867
  • Vector type
    Bacterial vector based on pMB1 replicon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Streptomycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    aadA
  • Insert Size (bp)
    2018

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The streptomycin/spectinomycin resistance cassette is derived from pBSL15-Omega (Lindemann, A. et al., FEMS Microbiol. Lett. 312:184-191, 2010)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGD-SmR was a gift from Hans-Martin Fischer (Addgene plasmid # 74109 ; http://n2t.net/addgene:74109 ; RRID:Addgene_74109)

  • For your References section:

    Versatile vectors for efficient mutagenesis of Bradyrhizobium diazoefficiens and other Alphaproteobacteria. Ledermann R, Strebel S, Kampik C, Fischer HM. Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15. 10.1128/AEM.04085-15 PubMed 26921431
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