pTETSIX
(Plasmid #74104)

• Purpose: mCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria
• Depositing Lab: Hans-Martin Fischer
• Publication: Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.

Backbone

• Vector backbone: pUCBM21
• Total vector size (bp): 6766
• Modifications to backbone: see Ledermann et al., Appl. Env. Microbiol., 2016
• Vector type: Conjugative bacterial vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tetA(C)
• Species: Synthetic
• Insert Size (bp): 1191
• Mutation: C-to-T transition at pos. no. 1049 of tetA(C) leading to T350I muation in TetA(C)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The source of tetA(C) is pBR322 (Bolivar, F. et al., Gene 2:95-113, 1977)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry