pREDSIX
(Plasmid #74103)

• Purpose: (Empty Backbone) mCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other Alphaproteobacteria
• Depositing Lab: Hans-Martin Fischer
• Publication: Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.

Backbone

• Vector backbone: pUCBM21
• Modifications to backbone: see Ledermann et al., Appl. Env. Microbiol., 2016
• Vector type: Conjugative bacterial vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 708

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The source of Ptuf-mCherry is plasmid pMRE-105-mCherry (Schada von Borzyskowski, L. et al., ACS Synth. Biol. 4:430-443 2015) which was kindly provided by Tobias Erb, MPI, Marburg, Germany
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry