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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]
(Plasmid #74101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSport I
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 4110
  • Total vector size (bp) 6923
  • Modifications to backbone
    Green Lantern Green Fluorescent Protein, a segment of rabbit beta-globin gene including the last intron and full length 3'UTR, and a dual chicken beta-globin hypersensitive site 4 (HS4) insulator element, and an NheI site were introduced into pSport 1.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Green Lantern Green Fluorescent Protein
  • Insert Size (bp)
    730

Gene/Insert 2

  • Gene/Insert name
    Rabbit beta 1-globin gene 3'UTR
  • Species
    O. cuniculus
  • Insert Size (bp)
    2184

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Green Lantern Green Fluorescent Protein from Life Technologies rabbit beta globin 3'UTR from RIchard Harland, U CA Berkeley

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2] was a gift from Ben Szaro (Addgene plasmid # 74101 ; http://n2t.net/addgene:74101 ; RRID:Addgene_74101)

  • For your References section:

    A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles. Wang C, Szaro BG. Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20. 10.1016/j.ydbio.2014.11.010 PubMed 25448690
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