pBluescriptII[attB/Ins1]
(Plasmid #74100)

• Purpose: "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)
• Depositing Lab: Ben Szaro
• Publication: Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20.

Backbone

• Vector backbone: pBluescript II SK(+)
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 3618
• Modifications to backbone: A small piece of pSPORT1 containing an attB element and a dual chicken beta-globin Hyper Sensitive Site 4 (HS4) insulator core elements was cloned into the ClaI/SpeI sites of pBluescript II SK (+)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Phi C31 attb element
• Insert Size (bp): 27

Gene/Insert 2

• Gene/Insert name: Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
• Species: G. gallus (chicken)
• Insert Size (bp): 510

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry