BAS285 C terminal mKO2 hygBR
(Plasmid
#74080)
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PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 4890
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemKO2
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SpeciesS. pombe (fission yeast)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BAS285 C terminal mKO2 hygBR was a gift from Bassem Al-Sady (Addgene plasmid # 74080 ; http://n2t.net/addgene:74080 ; RRID:Addgene_74080) -
For your References section:
Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics. Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD. PLoS One. 2016 Aug 1;11(8):e0159292. doi: 10.1371/journal.pone.0159292. eCollection 2016. PONE-D-16-08423 [pii] PubMed 27479698
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/plasmids/74/74080/74080-map_ASz7IbJRwEoJ.pdf.940x940_q85_autocrop.png)