pCRII-HSV2.3 TCR beta chain
(Plasmid #74077)

• Purpose: gBT-I TCR beta chain cDNA in pCRII
• Depositing Lab: Francis R. Carbone
• Publication: Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63.

Backbone

• Vector backbone: pCRII
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3947
• Total vector size (bp): 5009
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TCR beta cDNA clone HSV2.3
• Alt name: T cell receptor beta chain
• Species: M. musculus (mouse)
• Insert Size (bp): 1062
• Entrez Gene: Tcrb (a.k.a. TCRbeta, Tib)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the TCR beta chain cDNA from the HSV2.3 clone and was used to create the transgenic construct p3A9cbetaTCR.HSV2.3
(Addgene plasmid #69594).