pGEMT-T-HSV2.3 TCR alpha chain
(Plasmid #74076)

• Purpose: gBT-I TCR a chain cDNA in pGEM-T
• Depositing Lab: Francis R. Carbone
• Publication: Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63.

Backbone

• Vector backbone: pGEM-T
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 3852
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TCR alpha clone HSV2.3
• Alt name: T cell receptor alpha chain
• Species: M. musculus (mouse)
• Insert Size (bp): 847
• Entrez Gene: Tcra (a.k.a. Tcralpha)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the TCR alpha chain cDNA from the HSV2.3 clone and was used to create the transgenic construct pES.HSV2.3a
(Addgene plasmid #69593).