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Addgene

FH95pUP95S73DGW (F3)
(Plasmid #74040)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore (Addgene plasmid # 14883)
  • Backbone size w/o insert (bp) 9941
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Psd95
  • gRNA/shRNA sequence
    GGA CAT CCA GGC ACA CAA G
  • Species
    R. norvegicus (rat)
  • Mutation
    S73D
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BstBI (destroyed during cloning)
  • 5′ sequencing primer H1 tcgctatgtgttctgggaaa
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene did not confirm the presence of the S73D mutation.

Map: FHUGW-(pUb)-HinDIII-SalI-XbaI-(p95S73D)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH95pUP95S73DGW (F3) was a gift from Robert Malenka & Bernardo Sabatini & Weifeng Xu (Addgene plasmid # 74040 ; http://n2t.net/addgene:74040 ; RRID:Addgene_74040)

  • For your References section:

    Destabilization of the postsynaptic density by PSD-95 serine 73 phosphorylation inhibits spine growth and synaptic plasticity. Steiner P, Higley MJ, Xu W, Czervionke BL, Malenka RC, Sabatini BL. Neuron. 2008 Dec 10;60(5):788-802. doi: 10.1016/j.neuron.2008.10.014. 10.1016/j.neuron.2008.10.014 PubMed 19081375
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