FUP95Q15AGW (D5)
(Plasmid
#74028)
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Purposelentiviral expression of Psd95-Q15A-EGFP fusion
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Backbone manufacturerDavid Baltimore (Addgene plasmid # 14883)
- Backbone size w/o insert (bp) 9941
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePsd95
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SpeciesR. norvegicus (rat)
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MutationQ15A
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Entrez GeneDLG4 (a.k.a. MRD62, PSD95, SAP-90, SAP90)
- Promoter hUbC
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer hUBCpro-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Map: FUGW-HinDIII-SalI-XbaI-BamHI-SmaI-KpnI-EcoRI-XbaI-(P95Q15A)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUP95Q15AGW (D5) was a gift from Robert Malenka & Oliver Schluter & Weifeng Xu (Addgene plasmid # 74028 ; http://n2t.net/addgene:74028 ; RRID:Addgene_74028) -
For your References section:
Molecular dissociation of the role of PSD-95 in regulating synaptic strength and LTD. Xu W, Schluter OM, Steiner P, Czervionke BL, Sabatini B, Malenka RC. Neuron. 2008 Jan 24;57(2):248-62. doi: 10.1016/j.neuron.2007.11.027. 10.1016/j.neuron.2007.11.027 PubMed 18215622