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Addgene

FH95pUG+p97W (B8)
(Plasmid #74017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 74017 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    David Baltimore (Addgene plasmid # 14883)
  • Backbone size w/o insert (bp) 9941
  • Vector type
    Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Psd95 shRNA
  • Alt name
    EGFP - Sap97 (beta isoform) fusion co-expressed
  • gRNA/shRNA sequence
    GGA CAT CCA GGC ACA CAA G
  • Promoter H1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BstBI (destroyed during cloning)
  • 5′ sequencing primer H1 tcgctatgtgttctgggaaa
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Map: FHUG+W-SalI-XbaI-BamHI-(EGFP+)-BspEI-BglII-XhoI-SacI-HinDIII-BstBI-EcoRI-(p97 I3)-EcoRV-HinDIII-ClaI-FHUG+W

Also associated with Liu, et al. J Neurophysiol. 2014 Feb;111(3):648-58. doi: 10.1152/jn.00262.2013 PMID 24225540.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH95pUG+p97W (B8) was a gift from Robert Malenka & Oliver Schluter & Weifeng Xu (Addgene plasmid # 74017 ; http://n2t.net/addgene:74017 ; RRID:Addgene_74017)

  • For your References section:

    Alternative N-terminal domains of PSD-95 and SAP97 govern activity-dependent regulation of synaptic AMPA receptor function. Schluter OM, Xu W, Malenka RC. Neuron. 2006 Jul 6;51(1):99-111. 10.1016/j.neuron.2006.05.016 PubMed 16815335
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