FUp95GW (B7)
(Plasmid
#74016)
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Purposelentiviral expression of shRNA resistant Psd95-EGFP fusion
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 74016 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Backbone manufacturerDavid Baltimore (Addgene plasmid # 14883)
- Backbone size w/o insert (bp) 9941
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePsd95
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SpeciesR. norvegicus (rat)
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Entrez GeneDLG4 (a.k.a. MRD62, PSD95, SAP-90, SAP90)
- Promoter hUbC
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer hUBCpro-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Map: FUGW-HinDIII-SalI-XbaI-BamHI-SmaI-KpnI-EcoRI-XbaI-(p95)
PSD95 is isoform alpha.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUp95GW (B7) was a gift from Robert Malenka & Oliver Schluter & Weifeng Xu (Addgene plasmid # 74016 ; http://n2t.net/addgene:74016 ; RRID:Addgene_74016) -
For your References section:
Alternative N-terminal domains of PSD-95 and SAP97 govern activity-dependent regulation of synaptic AMPA receptor function. Schluter OM, Xu W, Malenka RC. Neuron. 2006 Jul 6;51(1):99-111. 10.1016/j.neuron.2006.05.016 PubMed 16815335