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pcDNA3.1-FLAG-SBP-STAG2 (V181M)
(Plasmid #73971)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73971 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 9379
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    STAG2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3951
  • Mutation
    V181M
  • GenBank ID
    NM_001042749
  • Entrez Gene
    STAG2 (a.k.a. SA-2, SA2, SCC3B, bA517O1.1)
  • Tag / Fusion Protein
    • FLAG-SBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-FLAG-SBP-STAG2 (V181M) was a gift from Todd Waldman (Addgene plasmid # 73971 ; http://n2t.net/addgene:73971 ; RRID:Addgene_73971)

  • For your References section:

    Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. Kim JS, He X, Orr B, Wutz G, Hill V, Peters JM, Compton DA, Waldman T. PLoS Genet. 2016 Feb 12;12(2):e1005865. doi: 10.1371/journal.pgen.1005865. eCollection 2016 Feb. PGENETICS-D-15-02663 [pii] PubMed 26871722
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