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Addgene

pMAZ-SK
(Plasmid #73962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCOLADuet
  • Backbone manufacturer
    Merck Millipore
  • Total vector size (bp) 2779
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA
  • gRNA/shRNA sequence
    aacgaaaccgtcgttgtagt
  • Promoter pTet

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tctggtcctcgagtctggtt
  • 3′ sequencing primer aggataccggtaggctgcat
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAZ-SK was a gift from Alex Nielsen (Addgene plasmid # 73962 ; http://n2t.net/addgene:73962 ; RRID:Addgene_73962)

  • For your References section:

    CRMAGE: CRISPR Optimized MAGE Recombineering. Ronda C, Pedersen LE, Sommer MO, Nielsen AT. Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452. 10.1038/srep19452 PubMed 26797514
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