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Addgene

pUAST attB BmOr2 (Y464A)
(Plasmid #73927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST attB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BmOr2 (Y464A)
  • Species
    Bombyx mori
  • Insert Size (bp)
    1000
  • Mutation
    changed 464 Tyrosine to Alanine
  • Promoter hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCasper-hs (5'-GCAACTACTGAAATCTGCCAAG-3')
  • 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAST attB BmOr2 (Y464A) was a gift from Leslie Vosshall (Addgene plasmid # 73927 ; http://n2t.net/addgene:73927 ; RRID:Addgene_73927)

  • For your References section:

    Amino acid residues contributing to function of the heteromeric insect olfactory receptor complex. Nakagawa T, Pellegrino M, Sato K, Vosshall LB, Touhara K. PLoS One. 2012;7(3):e32372. doi: 10.1371/journal.pone.0032372. Epub 2012 Mar 5. 10.1371/journal.pone.0032372 PubMed 22403649
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