pJKR-H-benM
(Plasmid
#73925)
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PurposeMuconate biosensor. Expresses GFP proportional to muconate concentration within the cell.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73925 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemuconate biosensing apparatus
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJKR-H-benM was a gift from George Church (Addgene plasmid # 73925 ; http://n2t.net/addgene:73925 ; RRID:Addgene_73925) -
For your References section:
Genetically encoded sensors enable real-time observation of metabolite production. Rogers JK, Church GM. Proc Natl Acad Sci U S A. 2016 Feb 8. pii: 201600375. 10.1073/pnas.1600375113 PubMed 26858408
