pCFD5
(Plasmid #73914)

• Purpose: multiplex gRNA plasmid
• Depositing Lab: Simon Bullock
• Publication: Port et al Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5.

Backbone

• Vector backbone: pValium22
• Backbone manufacturer: Norbert Perrimon, Jian-Quan Ni, Harvard
• Total vector size (bp): 6248
• Vector type: Insect Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dU6-3:tRNA:gRNA:tRNA:gRNA
• gRNA/shRNA sequence: empty
• Species: D. melanogaster (fly)
• Insert Size (bp): 750
• Promoter: dU6:3

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ACGTTTTATAACTTATGCCCCTAAG
• 3′ sequencing primer: GCCGAGCACAATTGTCTAGAATGC

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 30 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid.

Visit crisprflydesign.org for more information.