pFA6a-zeoCV
(Plasmid
#73890)
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Purposefission yeast drug selection marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73890 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6a-kanMX6
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Vector typeMammalian Expression, Bacterial Expression, Yeast Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Bleocin (Zeocin), 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namezeoCV
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Alt nameCMV/zeo
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SpeciesH. sapiens (human), S. cerevisiae (budding yeast), S. pombe (fission yeast)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer atcatgaccggtttaaacCGTTACATAACTTACGGTAA
- 3′ sequencing primer atccctaggctgcagatctGCAGTGAAAAAAATGCTTTA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-zeoCV was a gift from Kazunori Tomita (Addgene plasmid # 73890 ; http://n2t.net/addgene:73890 ; RRID:Addgene_73890) -
For your References section:
Sequential and counter-selectable cassettes for fission yeast. Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita K. BMC Biotechnol. 2016 Nov 8;16(1):76. 10.1186/s12896-016-0307-4 [pii] PubMed 27825338