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Addgene

pFA6a-neoCV
(Plasmid #73889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFA6a-kanMX6
  • Vector type
    Mammalian Expression, Bacterial Expression, Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    neoCV
  • Alt name
    CMV/neo
  • Species
    H. sapiens (human), S. cerevisiae (budding yeast), S. pombe (fission yeast)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PmeI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer atcatgaccggtttaaacCGTTACATAACTTACGGTAA
  • 3′ sequencing primer atccctaggctgcagatctGCAGTGAAAAAAATGCTTTA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-neoCV was a gift from Kazunori Tomita (Addgene plasmid # 73889 ; http://n2t.net/addgene:73889 ; RRID:Addgene_73889)

  • For your References section:

    Sequential and counter-selectable cassettes for fission yeast. Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita K. BMC Biotechnol. 2016 Nov 8;16(1):76. 10.1186/s12896-016-0307-4 [pii] PubMed 27825338
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