pEG03
(Plasmid #73841)

• Purpose: Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.
• Depositing Labs: Junghae Suh, Jeffrey Tabor
• Publication: Gomez et al ACS Nano. 2015 Nov 30.

Backbone

• Vector backbone: pNO001
• Backbone manufacturer: Dictybase (http://dictybase.org/)
• Backbone size w/o insert (bp): 6213
• Total vector size (bp): 9597
• Vector type: Dictyostelium discoideum expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: When expressing in Dictyostelium discoideum, the depositing laboratory recommends strain AX4. For dictyostelium, growth must be at 21-23 degrees Celsius.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PhyB(1-917) with C-term his tag
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2784
• Entrez Gene: PIL2 (a.k.a. AT3G62090, PHYTOCHROME-INTERACTING FACTOR 6, PIF6, phytochrome interacting factor 3-like 2)
• Tag / Fusion Protein:
  • 6x his tag (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dictybase (http://dictybase.org/) is the manufacturer of the backbone for pEG03.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry