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Addgene

pJK040 (pET-PurQ-A128T)
(Plasmid #73840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET24a
  • Backbone size w/o insert (bp) 5310
  • Total vector size (bp) 5980
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
  • Alt name
    purQ
  • Alt name
    BSU06470
  • Species
    Bacillus subtilis
  • Mutation
    A128T mutant
  • GenBank ID
    NP_388529
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Constructed by T. J. Kappock in the lab of J. Stubbe (MIT).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TEMPORARY: Am a little uncertain of the 3'-UTR region sequence between the XhoI site and stop codon. Original sequencing files were not available.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK040 (pET-PurQ-A128T) was a gift from T. J. Kappock & JoAnne Stubbe (Addgene plasmid # 73840 ; http://n2t.net/addgene:73840 ; RRID:Addgene_73840)

  • For your References section:

    The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation. Hoskins AA, Anand R, Ealick SE, Stubbe J. Biochemistry. 2004 Aug 17;43(32):10314-27. 10.1021/bi049127h PubMed 15301530
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