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Plekhm1- PH1 pGEX 4T2-GST
(Plasmid #73833)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4970
  • Total vector size (bp) 5370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plekhm1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Mutation
    to vector was cloned PH1 domain of human Plekhm1 (aa: Q513-V638)
  • Entrez Gene
    PLEKHM1 (a.k.a. AP162, B2, OPTA3, OPTB6)
  • Tag / Fusion Protein
    • Gst (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer pGex-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Plekhm1- PH1 pGEX 4T2-GST was a gift from Paul Odgren (Addgene plasmid # 73833 ; http://n2t.net/addgene:73833 ; RRID:Addgene_73833)
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